Technical Services The LilaLab will occasionally perform analyses on our specialized scientific instrumentation on a fee-for-service basis to outside contractors or research teams. Established rates, subject to change, are intended to cover the costs of lab instrument maintenance, disposables, solvents and other chemicals, and time for LilaLab personnel to perform sample runs and analysis. Contact: mlila@ncsu.edu, 704-250-5407 High Pressure Liquid Chromatography (HPLC) Instrument Specifications Agilent 1260 Infinity HPLC equipped with DAD and ELSD detectors Agilent 1200 HPLC equipped with DAD and RID detectors Agilent 1200 HPLC equipped with DAD and FLD detectors Anthocyanin analysis Includes identification of peaks by comparison to standards and quantification of total anthocyanins as cyanidin-3-O-glucoside equivalents based on peak area measurements Flavonoid and phenolic acids analyses Identification against available standards using DAD detector Proanthocyanidin analysis HPLC on normal phase column with fluorescence detection according to degree of polymerization. Quantification based on total peak areas PDF of chromatogram with peak areas, identification against common available standards, and quantitation if required User Rates Personal communication per project Liquid Chromatography-Mass Spectrometers (LC-MS and LC-MSn ) Instrument Specifications Waters Xevo G2-XS QTof Quadrupole Time-of-Flight Mass Spectrometry Shimadzu LCMS-IT-TOF (m/z 150 – 2500, 1 ppm error) Agilent QTOF G6510A (m/z 150 – 2500, 5 ppm error) User Rates Sample Analysis – $400 per sample (Industry), $250 (Academic) Includes sample preparation, full scan of ions in positive and negative modes with MS/MS, data analysis and interpretation Gas Chromatography-Mass Spectrometers Instrument Specifications Agilent 7890 with 5975C inert XL EI/CI MSD triple axis detector User Rates Personal communication per project For volatile samples only (no derivatization) Includes method development and identification from NIST library Circular Dichroism Spectrometer Instrument Specifications Jasco J-815 Cd Spectrometer User Rates Sample Analysis – costs negotiated based on sample type Includes scan of sample, no data analysis Polarimeter Instrument Specifications Jasco P-2000 Polarimeter User Rates Sample Analysis – $50 per sample Includes scan of sample, no data analysis Grace Reveleris® Prep purification system Instrument Specifications Both flash and preparative isolation of small molecules. Max pressure 1700 bar User Rates Personal communication per project High-Performance Counter-Current Chromatography Instrument Specifications Dynamic Extractions Ltd – Midi-HPCCC with Armen high-pressure pump equipped with UV/Vis detector User Rates Personal communication per project
