Technical Services The LilaLab has expertise with the following suite of in-house instrumentation which can be utilized in cooperative research proposals. High Pressure Liquid Chromatography (HPLC) Instrument Specifications Agilent 1260 Infinity HPLC equipped with DAD and ELSD detectors Agilent 1200 HPLC equipped with DAD and RID detectors Agilent 1200 HPLC equipped with DAD and FLD detectors Anthocyanin analysis Includes identification of peaks by comparison to standards and quantification of total anthocyanins as cyanidin-3-O-glucoside equivalents based on peak area measurements Flavonoid and phenolic acids analyses Identification against available standards using DAD detector Proanthocyanidin analysis HPLC on normal phase column with fluorescence detection according to degree of polymerization. Quantification based on total peak areas PDF of chromatogram with peak areas, identification against common available standards, and quantitation if required Liquid Chromatography-Mass Spectrometers (LC-MS and LC-MSn ) Instrument Specifications Waters Xevo G2-XS QTof Quadrupole Time-of-Flight Mass Spectrometry Shimadzu LCMS-IT-TOF (m/z 150 – 2500, 1 ppm error) Agilent QTOF G6510A (m/z 150 – 2500, 5 ppm error) Gas Chromatography-Mass Spectrometers Instrument Specifications Agilent 7890 with 5975C inert XL EI/CI MSD triple axis detector Including method development and identification from NIST library Circular Dichroism Spectrometer Instrument Specifications Jasco J-815 Cd Spectrometer Polarimeter Instrument Specifications Jasco P-2000 Polarimeter Grace Reveleris® Prep purification system Instrument Specifications Both flash and preparative isolation of small molecules. Max pressure 1700 bar High-Performance Counter-Current Chromatography Instrument Specifications Dynamic Extractions Ltd – Midi-HPCCC with Armen high-pressure pump equipped with UV/Vis detector
